Isolation of simple sequence repeats from groundnut
Electron. j. biotechnol
;
10(3): 473-480, July 2007. ilus, graf, tab
Artículo
en Inglés
| LILACS
| ID: lil-640488
ABSTRACT
SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR inserts.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Idioma:
Inglés
Revista:
Electron. j. biotechnol
Asunto de la revista:
Biotecnologia
Año:
2007
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
China
Institución/País de afiliación:
Shandong Peanut Research Institute/CN
Similares
MEDLINE
...
LILACS
LIS