Cloning and analysis of a NBS-LRR disease resistance gene candidate PnAG1 from peanut (Arachis hypogaea L. )
Electron. j. biotechnol
;
14(6): 6-6, Nov. 2011. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-640523
ABSTRACT
Background:
Based on the conserved sequences of a known NBS resistance gene, a pair of degenerate primers was designed to amplify the NBS-LRR resistance gene from peanut using PCR and RACE methods.Results:
Analyzing the amino acid sequence by BLAST on NCBI, which was deduced from the 1088bp-long gene named PnAG1-2, showed that it had a certain homology with some resistance proteins, among which Arachis cardenasii resistance protein gene had the highest homology (66 percent). Relative quantification PCR analysis indicated that PnAG1-2 gene expresses more in J11 (an A. flavus-resistant variety) than in JH1012 (an A. flavus-susceptible variety) when the harvest time was coming.Conclusions:
In this study, the NBS-LRR resistance sequence was successfully cloned from peanut and prokaryotic expression was done on the gene, which provided a foundation for cultivating anti-A. flavus peanut varieties.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Arachis
/
Enfermedades de las Plantas
/
Genes de Plantas
/
Inmunidad Innata
Idioma:
Inglés
Revista:
Electron. j. biotechnol
Asunto de la revista:
Biotecnologia
Año:
2011
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
China
Institución/País de afiliación:
Shandong Academy of Agricultural Sciences/CN
/
Shandong Peanut Research Institute/CN
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