Intrinsically bent DNA in replication origins and gene promoters
Genet. mol. res. (Online)
; 7(2): 549-558, 2008. ilus
Article
en En
| LILACS
| ID: lil-640982
Biblioteca responsable:
BR26.1
ABSTRACT
Intrinsically bent DNA is an alternative conformation of the DNA molecule caused by the presence of dA/dT tracts, 2 to 6 bp long, in a helical turn phase DNA or with multiple intervals of 10 to 11 bp. Other than flexibility, intrinsic bending sites induce DNA curvature in particular chromosome regions such as replication origins and promoters. Intrinsically bent DNA sites are important in initiating DNA replication, and are sometimes found near to regions associated with the nuclear matrix. Many methods have been developed to localize bent sites, for example, circular permutation, computational analysis, and atomic force microscopy. This review discusses intrinsically bent DNA sites associated with replication origins and gene promoter regions in prokaryote and eukaryote cells. We also describe methods for identifying bent DNA sites for circular permutation and computational analysis.
Palabras clave
Texto completo:
1
Índice:
LILACS
Asunto principal:
ADN
/
Regiones Promotoras Genéticas
/
Origen de Réplica
/
Conformación de Ácido Nucleico
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Genet. mol. res. (Online)
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2008
Tipo del documento:
Article