Rhinovirus detection using different PCR-based strategies
Braz. j. microbiol
;
43(2): 739-743, Apr.-June 2012. tab
Artículo
en Inglés
| LILACS
| ID: lil-644491
ABSTRACT
Human rhinoviruses (HRVs) are the major cause of the common cold. HRVs were recently reclassified into the Enterovirus genus (HEV) in the Picornaviridae family. HRVs and other members of the HEV genus share many common features, including sense RNA genomes and partial nucleotide sequence identity. The aim of this study was to evaluate different HRV detection strategies. Samples from adults with acute respiratory infection (n = 291) who were treated in Sao Paulo Hospital (2001-2003) were tested using three assays. The first assay detected picornaviruses by RT-PCR and hybridization, the second detected rhinoviruses using RT-PCR/sequencing, and the third differentiated HRV from HEV using duplex semi-nested-RT-PCR. Analysis of the results obtained from the first two strategies revealed 83% concordance. Discordant samples were then evaluated by the third protocol, and 82% were negative. The picornavirus detection protocol was more sensitive but less specific than the rhinovirus detection protocols. The semi-nested protocol utilized in the present study was less sensitive and was not useful in differentiating HRV from HEV. Sequencing assays examining different genes would address the best strategy of confirming rhinovirus and enterovirus infections.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Picornaviridae
/
Infecciones del Sistema Respiratorio
/
Rhinovirus
/
Técnicas In Vitro
/
Secuencia de Bases
/
Reacción en Cadena de la Polimerasa
/
Genoma Viral
/
Resfriado Común
/
Infecciones por Picornaviridae
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Tipo de estudio:
Estudio diagnóstico
/
Estudios de evaluación
Límite:
Humanos
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2012
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal de Sao Paulo/BR
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