Evaluation of rapid tests for human immunodeficiency virus as a tool to detect recent seroconversion
Braz. j. infect. dis
;
16(5): 452-456, Sept.-Oct. 2012. tab
Artículo
en Inglés
| LILACS
| ID: lil-653434
ABSTRACT
The identification of recent HIV infection is important for epidemiological studies and to monitor the epidemic. The objective of this study was to evaluate two rapid tests that are easily available to the Brazilian scientific community for using as markers of recent HIV infection. The Rapid Test - HIV-1/2 Bio-Manguinhos (Bio-Manguinhos/Fiocruz, Brazil) and the Rapid Check HIV 1&2 (NDI-UFES, Center for Infectious Diseases, Universidade Federal do Espírito Santo) were tested, using 489 samples with HIV positive serology, from blood donors, previously classified as recent or long-term infection by serological testing algorithm for recent HIV seroconversion (STARHS) or LS-HIV Vitros assay methods. The samples were diluted prior to testing (1:50 and 1:100 for the Rapid Test - HIV-1/2 Bio-Manguinhos, and 1:500 and 1:600 for the Rapid Check HIV 1&2). Negative samples were considered recent infection, whereas those showing any color intensity were associated with long-term infection. The best dilutions were 1:100 for HIV-1/2 Bio-Manguinhos test (Kappa = 0.840; overall agreement = 0.93), and 1:500 for the Rapid Check HIV 1&2 (Kappa = 0.867; overall agreement = 0.94). The results suggest that both rapid tests can be used to detect recent seroconversion.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Juego de Reactivos para Diagnóstico
/
Anticuerpos Anti-VIH
/
Infecciones por VIH
/
VIH-1
/
VIH-2
Tipo de estudio:
Estudio diagnóstico
/
Estudios de evaluación
/
Estudio pronóstico
Límite:
Humanos
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Braz. j. infect. dis
Asunto de la revista:
Enfermedades Transmisibles
Año:
2012
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Fundação Centro de Hematologia e Hemoterapia de Minas Gerais/BR
/
Fundação Hemope/BR
/
Fundação Pró-Sangue Hemocentro de São Paulo/BR
/
Instituto Estadual de Hematologia Arthur de Siqueira Cavalcanti/BR
/
Universidade Federal de São Paulo/BR
/
Universidade de São Paulo/BR
Similares
MEDLINE
...
LILACS
LIS