Shigella in Brazilian children with acute diarrhoea: prevalence, antimicrobial resistance and virulence genes
Mem. Inst. Oswaldo Cruz
;
108(1): 30-35, Feb. 2013. tab
Artículo
en Inglés
| LILACS
| ID: lil-666040
ABSTRACT
Diarrhoeal disease is still considered a major cause of morbidity and mortality among children. Among diarrhoeagenic agents, Shigella should be highlighted due to its prevalence and the severity of the associated disease. Here, we assessed Shigella prevalence, drug susceptibility and virulence factors. Faeces from 157 children with diarrhoea who sought treatment at the Children's Hospital João Paulo II, a reference children´s hospital in Belo Horizonte, state of Minas Gerais, Brazil, were cultured and drug susceptibility of the Shigella isolates was determined by the disk diffusion technique. Shigella virulence markers were identified by polymerase chain reaction. The bacterium was recovered from 10.8% of the children (88.2% Shigella sonnei). The ipaH, iuc, sen and ial genes were detected in strains isolated from all shigellosis patients; set1A was only detected in Shigella flexneri. Additionally, patients were infected by Shigella strains of different ial, sat, sen and set1A genotypes. Compared to previous studies, we observed a marked shift in the distribution of species from S. flexneri to S. sonnei and high rates of trimethoprim/sulfamethoxazole resistance.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Shigella
/
Factores de Virulencia
/
Diarrea
/
Disentería Bacilar
Tipo de estudio:
Estudio diagnóstico
/
Estudio de prevalencia
/
Estudio pronóstico
/
Factores de riesgo
Límite:
Child, preschool
/
Femenino
/
Humanos
/
Lactante
/
Masculino
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2013
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal de Minas Gerais/BR
Similares
MEDLINE
...
LILACS
LIS