Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil
Rev. Soc. Bras. Med. Trop
;
46(1): 97-99, Jan.-Feb. 2013. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-666813
ABSTRACT
INTRODUCTION:
West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil.METHODS:
Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus.RESULTS:
WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses.CONCLUSIONS:
This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Virus del Nilo Occidental
/
Proteínas Recombinantes
/
Proteínas del Envoltorio Viral
/
Anticuerpos Neutralizantes
/
Anticuerpos Antivirales
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Rev. Soc. Bras. Med. Trop
Asunto de la revista:
Medicina Tropical
Año:
2013
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade de São Paulo/BR
Similares
MEDLINE
...
LILACS
LIS