Ischemic preconditioning and the gene expression of enteric endothelial cell biology of rats submitted to intestinal ischemia and reperfusion
Acta cir. bras
;
28(3): 167-173, Mar. 2013. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-667925
ABSTRACT
PURPOSE:
To investigate the effects of ischemic preconditioning (IPC) on the expression of pro and anti-apoptotic genes in rat endothelial cells undergoing enteric ischemia (I) and reperfusion (R).METHODS:
Thirty rats underwent clamping of the superior mesenteric vessels. Sham group (GS) laparotomy only; Ischemia (GI) intestinal ischemia (60 min); Ischemia and Reperfusion (GIR) ischemia (60 min) and reperfusion (120 min); Ischemia and intestinal ischemic preconditioning (GI + IPC) 5 minutes of ischemia followed by 10 min of reperfusion before sustained ischemia (60 min) ischemia and reperfusion and IPC (GIR + IPC) 5 min ischemia followed by 10 min of reperfusion before sustained ischemia (60min) and reperfusion (120 min). Rat Endothelial Cell Biology (PCR array) to determine the expression of genes related to endothelial cell biology.RESULTS:
Gene expression of pro-apoptotic markers (Casp1, Casp6, Cflar, Fas, and Pgl) was down regulated in GI+IPC and in GIR + IPC. In contrast, the expression of anti-apoptotic genes (Bcl2 and Naip2), was up-regulated in GI + IPC and in GIR + IPC.CONCLUSION:
Ischemic preconditioning may protect against cell death caused by ischemia and reperfusion.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Daño por Reperfusión
/
Expresión Génica
/
Apoptosis
/
Precondicionamiento Isquémico
/
Células Endoteliales
/
Intestinos
Límite:
Animales
Idioma:
Inglés
Revista:
Acta cir. bras
Asunto de la revista:
Cirugía General
/
Procedimentos Cir£rgicos Operat¢rios
Año:
2013
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
UNIFESP/BR
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