Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection
Rev. Soc. Bras. Med. Trop
;
46(2): 161-165, Mar-Apr/2013. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-674638
ABSTRACT
INTRODUCTION:
Human adenoviruses (HAdV) play an important role in the etiology of severe acute lower respiratory infection, especially in immunocompromised individuals. The aim of the present study was detect the HAdV through differentmethods:
direct fluorescence assay (DFA) and nested-polymerase chain reaction (PCR-nested) from patients with acute respiratory infection (ARI) up to 7 days of symptoms onset.METHODS:
Samples (n=643) were collected from different risk groups during from 2001 to 2010 139 adults attended in an Emergency Room Patients (ERP); 205 health care workers (HCW); 69 from Renal Transplant Outpatients (RTO); 230 patients in hematopoietic stem cell transplantation (HSCT) program.RESULTS:
Among all patients (n=643) adenovirus was detected on 13.2% by DFA and/or PCR 6/139 (4.3%) adults from ERP, 7/205 (3.4%) from HCW samples, 4/69 (5.8%) from RTO and 68/230 (29.5%) from HSCT patients. Nested PCR showed higher detection (10%) compared to DFA test (3.8%) (p < 0.001). HSCT patients presented significantly higher prevalence of HAdV infection.CONCLUSIONS:
Adenovirus detection through nested-PCR assay was higher. However the inclusion of molecular method in laboratorial routine diagnostic should be evaluated considering the reality of each specific health service. .
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Infecciones del Sistema Respiratorio
/
Infecciones por Adenovirus Humanos
Tipo de estudio:
Estudio diagnóstico
/
Estudio de prevalencia
/
Factores de riesgo
Límite:
Adolescente
/
Adulto
/
Anciano
/
Aged80
/
Niño
/
Femenino
/
Humanos
/
Masculino
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Rev. Soc. Bras. Med. Trop
Asunto de la revista:
Medicina Tropical
Año:
2013
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal de São Paulo/BR
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