Your browser doesn't support javascript.
loading
Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures and concentrations of sodium chloride
Lee, H. Y; Chai, L. C; Pui, C. F; Mustafa, S; Cheah, Y. K; Nishibuchi, M; Radu, S.
  • Lee, H. Y; Universiti Putra Malaysia. Faculty of Food Science and Technology. Centre of Excellence for Food Safety Research. Serdang. MY
  • Chai, L. C; Universiti Malaya. Faculty of Science. Department of Microbiology. Kuala Lumpur. MY
  • Pui, C. F; Universiti Putra Malaysia. Faculty of Food Science and Technology. Centre of Excellence for Food Safety Research. Serdang. MY
  • Mustafa, S; Universiti Putra Malaysia. Faculty of Biotechnology and Biomolecular Science. Department of Molecular and Cellular Biology. Serdang. MY
  • Cheah, Y. K; Universiti Putra Malaysia. Faculty of Medicine and Health Sciences. Department of Biomedical Sciences. Serdang. MY
  • Nishibuchi, M; Kyoto University. Center for Southeast Asian Studies. Yoshida. JP
  • Radu, S; Universiti Putra Malaysia. Faculty of Food Science and Technology. Centre of Excellence for Food Safety Research. Serdang. MY
Braz. j. microbiol ; 44(1): 51-55, 2013. ilus, tab
Artículo en Inglés | LILACS | ID: lil-676886
ABSTRACT
Biofilm formation can lead to various consequences in the food processing line such as contamination and equipment breakdowns. Since formation of biofilm can occur in various conditions; this study was carried out using L. monocytogenes ATCC 19112 and its biofilm formation ability tested under various concentrations of sodium chloride and temperatures. Cultures of L. monocytogenes ATCC 19112 were placed in 96-well microtitre plate containing concentration of sodium chloride from 1-10% (w/v) and incubated at different temperature of 4 ºC, 30 ºC and 45 ºC for up to 60 h. Absorbance reading of crystal violet staining showed the density of biofilm formed in the 96-well microtitre plates was significantly higher when incubated in 4 ºC. The formation of biofilm also occurs at a faster rate at 4 ºC and higher optical density (OD 570 nm) was observed at 45 ºC. This shows that storage under formation of biofilm that may lead to a higher contamination along the processing line in the food industry. Formation of biofilm was found to be more dependent on temperature compared to sodium chloride stress.
Asunto(s)


Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Coloración y Etiquetado / Cloruro de Sodio / Contaminación de Alimentos / Espectrofotómetros / Biopelículas / Incubadoras / Listeria monocytogenes Límite: Humanos Idioma: Inglés Revista: Braz. j. microbiol Asunto de la revista: Microbiologia Año: 2013 Tipo del documento: Artículo País de afiliación: Japón / Malasia Institución/País de afiliación: Kyoto University/JP / Universiti Malaya/MY / Universiti Putra Malaysia/MY

Similares

MEDLINE

...
LILACS

LIS


Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Coloración y Etiquetado / Cloruro de Sodio / Contaminación de Alimentos / Espectrofotómetros / Biopelículas / Incubadoras / Listeria monocytogenes Límite: Humanos Idioma: Inglés Revista: Braz. j. microbiol Asunto de la revista: Microbiologia Año: 2013 Tipo del documento: Artículo País de afiliación: Japón / Malasia Institución/País de afiliación: Kyoto University/JP / Universiti Malaya/MY / Universiti Putra Malaysia/MY