EsxA might as a virulence factor induce antibodies in patients with Staphylococcus aureus infection
Braz. j. microbiol
;
44(1): 267-271, 2013. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-676912
ABSTRACT
Staphylococcus aureus (S. aureus) is an important human pathogen, which commonly causes the acquired infectious diseases in the hospital and community. Effective and simple antibiotic treatment against S. aureus-related disease becomes increasingly difficult. Developing a safe and effective vaccine against S. aureus has become one of the world's hot spots once again. The key issue of developing the vaccine of S. aureus is how to find an ideal key pathogenic gene of S. aureus. It was previously suggested that EsxA might be a very important factor in S. aureus abscess formation in mice, but clinical experimental evidence was lacking. We therefore expressed EsxA protein through prokaryotic expression system and purified EsxA protein by Ni-affinity chromatography. ELISA was used to detect the anti-EsxA antibodies in sera of 78 patients with S. aureus infection and results showed that the anti-EsxA antibodies were positive in the sera of 19 patients. We further analyzed the EsxA positive antibodies related strains by antimicrobial susceptibility assay and found that all of the corresponding strains were multi-drug resistant. Among those multi-drug resistant strains, 73.7% were resistant to MRSA. The results indicated EsxA is very important in the pathogenesis of S. aureus. We suggested that the EsxA is very valuable as vaccine candidate target antigens for prevention and control of S. aureus infection.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Infecciones Estafilocócicas
/
Staphylococcus aureus
/
Farmacorresistencia Microbiana
/
Vacunas
/
Susceptibilidad a Enfermedades
/
Antibacterianos
Tipo de estudio:
Estudios de evaluación
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2013
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
China
Institución/País de afiliación:
Jiangsu University/CN
/
The Second Affiliated Hospital of Soochow University/CN
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