Molecular identification of Giardia duodenalis in Ecuador by polymerase chain reaction-restriction fragment length polymorphism
Mem. Inst. Oswaldo Cruz
;
108(4): 512-515, jun. 2013. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-678280
ABSTRACT
The aim of this study was to determine the genetic diversity of Giardia duodenalis present in a human population living in a northern Ecuadorian rain forest. All Giardia positive samples (based on an ELISA assay) were analysed using a semi-nested polymerase chain reaction-restriction fragment length polymorphism assay that targets the glutamate dehydrogenase (gdh) gene; those amplified were subsequently genotyped using NlaIV and RsaI enzymes. The gdh gene was successfully amplified in 74 of 154 ELISA positive samples; 69 of the 74 samples were subsequently genotyped. Of these 69 samples, 42 (61%) were classified as assemblage B (26 as BIII and 16 as BIV), 22 (32%) as assemblage A (3 as AI and 19 as AII) and five (7%) as mixed AII and BIII types. In this study site we observe similar diversity in genotypes to other regions in Latin America, though in contrast to some previous studies, we found similar levels of diarrheal symptoms in those individuals infected with assemblage B compared with those infected with assemblage A.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Giardiasis
/
Giardia
/
Glutamato Deshidrogenasa
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
País/Región como asunto:
America del Sur
/
Ecuador
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2013
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Ecuador
Institución/País de afiliación:
Universidad Central del Ecuador/EC
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