Increasing the rate of drying reduces metabolic imbalance, lipid peroxidation and critical water content in radicles of garden pea (Pisum sativum L.)
Biol. Res
;
46(2): 121-130, 2013. ilus
Artículo
en Inglés
| LILACS
| ID: lil-683988
ABSTRACT
Orthodox seeds become desiccation-sensitive as they undergo germination. As a result, germinating seeds serve as a model to study desiccation sensitivity in plant tissues. The effects of the rate of drying on the viability, respiratory metabolism and free radical processes were thus studied during dehydration and wet storage of radicles of Pisum sativum. For both drying regimes desiccation could be described by exponential and inverse modified functions. Viability, as assessed by germination capacity and tetrazolium staining, remained at 100% during rapid (< 24 h) desiccation. However, it declined sharply at c. 0.26 g g¹ dm following slow (c. 5 days) drying. Increasing the rate of dehydration thus lowered the critical water content for survival. Rapid desiccation was also associated with higher activities and levels of malate dehydrogenase and the oxidized form of nicotinamide adenine dinucleotide. It was also accompanied by lower hydroperoxide levels and membrane damage. In addition, the activitiy of glutathione reductase was greater during rapid drying. Ageing may have contributed to increased damage during slow dehydration, since viability declined even in wet storage after two weeks. The results presented are consistent with rapid desiccation reducing the accumulation of damage resulting from desiccation-induced aqueous-based deleterious reactions. In addition, they show that radicles are a useful model to study desiccation sensitivity in plant tissues.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Semillas
/
Agua
/
Peroxidación de Lípido
/
Raíces de Plantas
/
Pisum sativum
/
Desecación
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2013
Tipo del documento:
Artículo
País de afiliación:
Sudáfrica
Institución/País de afiliación:
University of KwaZulu-Natal/ZA
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