Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
Rev. Soc. Bras. Med. Trop
; 46(5): 625-628, Sept-Oct/2013. tab, graf
Article
en En
| LILACS
| ID: lil-691413
Biblioteca responsable:
BR1.1
ABSTRACT
Introduction Herpes simplex virus (HSV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fluid samples has allowed the detection of viral DNA with high sensitivity and specificity. Methods Serial dilutions of quantified commercial controls of each virus were subjected to an in-house nested-PCR technique. Results The minimum detection limits for HSV and VZV were 5 and 10 copies/µL, respectively. Conclusions The detection limit of nested-PCR for HSV and VZV in this study was similar to the limits found in previous studies. .
Palabras clave
Texto completo:
1
Índice:
LILACS
Asunto principal:
ADN Viral
/
Reacción en Cadena de la Polimerasa
/
Simplexvirus
/
Herpes Simple
/
Herpes Zóster
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Rev. Soc. Bras. Med. Trop
Asunto de la revista:
MEDICINA TROPICAL
Año:
2013
Tipo del documento:
Article