A simple, rapid and economic method for detecting multidrug-resistant tuberculosis
Braz. j. infect. dis
;
17(6): 667-671, Nov.-Dec. 2013. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-696968
ABSTRACT
OBJECTIVE:
To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis.METHODS:
Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 isolates were sensitive to isonicotinylhydrazine and rifampin. Primers were designed to target five mutations hotspots that confer resistance to the first-line drugs isoniazid and rifampin, and multiplex allele specific polymerase chain reaction was performed. Whole-genome sequencing confirmed drug resistance mutations identified by multiplex allele specific polymerase chain reaction.RESULTS:
DNA sequencing revealed that 68.9% of multidrug-resistant strains have point mutations at codon 315 of the katG gene, 19.8% within the mabA-inhA promoter, and 98.0% at three hotspots within rpoB. Multiplex allele specific polymerase chain reaction detected each of these five mutations, yielding 82.3% sensitivity and 100% specificity for isoniazid resistance, and 97.9% sensitivity and 100% specificity for rifampin resistance as compared to drug susceptibility testing.CONCLUSIONS:
The results show that multiplex allele specific polymerase chain reaction is an inexpensive and practical method for rapid detection of multidrug-resistant tuberculosis in developing countries.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Tuberculosis Resistente a Múltiples Medicamentos
/
Reacción en Cadena de la Polimerasa Multiplex
/
Mycobacterium tuberculosis
/
Antituberculosos
Tipo de estudio:
Estudio diagnóstico
/
Evaluación Económica en Salud
/
Estudio pronóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
Braz. j. infect. dis
Asunto de la revista:
Enfermedades Transmisibles
Año:
2013
Tipo del documento:
Artículo
País de afiliación:
China
Institución/País de afiliación:
Xinxiang Medical University/CN
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