Application of statistical experimental design for optimisation of bioinsecticides production by sporeless Bacillus thuringiensis strain on cheap medium
Braz. j. microbiol
;
44(3): 927-933, July-Sept. 2013. graf, tab
Artículo
en Inglés
| LILACS
| ID: lil-699824
ABSTRACT
In order to overproduce bioinsecticides production by a sporeless Bacillus thuringiensis strain, an optimal composition of a cheap medium was defined using a response surface methodology. In a first step, a Plackett-Burman design used to evaluate the effects of eight medium components on delta-endotoxin production showed that starch, soya bean and sodium chloride exhibited significant effects on bioinsecticides production. In a second step, these parameters were selected for further optimisation by central composite design. The obtained results revealed that the optimum culture medium for delta-endotoxin production consists of 30 g L-1 starch, 30 g L-1 soya bean and 9g L-1 sodium chloride. When compared to the basal production medium, an improvement in delta-endotoxin production up to 50% was noted. Moreover, relative toxin yield of sporeless Bacillus thuringiensis S22 was improved markedly by using optimised cheap medium (148.5 mg delta-endotoxins per g starch) when compared to the yield obtained in the basal medium (94.46 mg delta-endotoxins per g starch). Therefore, the use of optimised culture cheap medium appeared to be a good alternative for a low cost production of sporeless Bacillus thuringiensis bioinsecticides at industrial scale which is of great importance in practical point of view.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Bacillus thuringiensis
/
Proteínas Bacterianas
/
Biotecnología
/
Reactores Biológicos
/
Medios de Cultivo
/
Endotoxinas
/
Proteínas Hemolisinas
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2013
Tipo del documento:
Artículo
País de afiliación:
Túnez
Institución/País de afiliación:
University of Sfax/TN
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