Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury
Acta cir. bras
;
29(3): 186-192, 03/2014. graf
Artículo
en Inglés
| LILACS
| ID: lil-703525
ABSTRACT
To determine the gene expression profile associated with oxidative stress and antioxidant defense in the lung tissue of mice subjected to intestinal ischemia and reperfusion. METHODS:
Twelve male, inbred mice (C57BL/6) were randomly assigned to one of two groups. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes; the ischemia/reperfusion group (IRG) was subjected to anesthesia, laparotomy, and ischemia of the small intestine for 60 minutes and to 60 minutes of reperfusion. A pool of six mice from each group was subjected to a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the oxidative stress and antioxidant defense genes. All genes that were up-regulated or down-regulated greater than three-fold, based on the algorithm 2.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Estrés Oxidativo
/
Isquemia
/
Pulmón
Límite:
Animales
Idioma:
Inglés
Revista:
Acta cir. bras
Asunto de la revista:
Cirugía General
/
Procedimentos Cir£rgicos Operat¢rios
Año:
2014
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Centro Universitário São Camilo/BR
/
Universidade Federal da Grande Dourados/BR
/
Universidade Federal de São Paulo/BR
/
Universidade de Mato Grosso do Sul/BR
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