CIAPIN1 gene silencing enhances chemosensitivity in a drug-resistant animal model in vivo
Braz. j. med. biol. res
;
47(4): 273-278, 8/4/2014. graf
Artículo
en Inglés
| LILACS
| ID: lil-705769
ABSTRACT
Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in vivo. We used lentivirus-vector-based RNAi to knock down CIAPIN1 in nude mice bearing MDR breast cancer tumors and found that lentivirus-vector-mediated silencing of CIAPIN1 could efficiently and significantly inhibit tumor growth when combined with chemotherapy in vivo. Furthermore, Western blot analysis showed that both CIAPIN1 and P-glycoprotein expression were efficiently downregulated, and P53 was upregulated, after RNAi. Therefore, we concluded that lentivirus-vector-mediated RNAi targeting of CIAPIN1 is a potential approach to reverse MDR of breast cancer. In addition, CIAPIN1 may participate in MDR of breast cancer by regulating P-glycoprotein and P53 expression.
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Neoplasias de la Mama
/
Doxorrubicina
/
Resistencia a Antineoplásicos
/
Silenciador del Gen
/
Péptidos y Proteínas de Señalización Intracelular
/
Antibióticos Antineoplásicos
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
2014
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
China
Institución/País de afiliación:
Harbin Medical University/CN
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