Genetic diversity and antimicrobial resistance in Streptococcus agalactiae strains recovered from female carriers in the Bucharest area
Mem. Inst. Oswaldo Cruz
;
109(2): 189-196, abr. 2014. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-705824
ABSTRACT
For the first time, we used multilocus sequence typing (MLST) to understand how Romanian group B streptococcus (GBS) strains fit into the global GBS population structure. Colonising isolates recovered from adult human females were tested for antibiotic resistance, were molecularly serotyped based on the capsular polysaccharide synthesis (cps) gene cluster and further characterised using a set of molecular markers (surface protein genes, pilus-encoded islands and mobile genetic elements inserted in the scpB-lmb intergenic region). Pulsed-field gel electrophoresis was used to complement the MLST clonal distribution pattern of selected strains. Among the 55 strains assigned to six cps types (Ia, Ib, II-V), 18 sequence types (STs) were identified by MLST. Five STs represented new entries to the MLST database. The prevalent STs were ST-1, ST-17, ST-19 and ST-28. Twenty molecular marker profiles were identified. The most common profiles (rib+GBSi1+PI-1, rib+GBSi1+PI-1, PI-2b and alp2/3+PI-1, PI-2a) were associated with the cps III/ST-17 and cps V/ST-1 strains. A cluster of fluoroquinolone-resistant strains was detected among the cps V/ST-19 members; these strains shared alp1 and IS1548 and carried PI-1, PI-2a or both. Our results support the usefulness of implementing an integrated genotyping system at the reference laboratory level to obtain the reliable data required to make comparisons between countries.
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Índice:
LILACS (Américas)
Asunto principal:
Streptococcus agalactiae
/
Variación Genética
/
ADN Bacteriano
/
Farmacorresistencia Microbiana
/
Portador Sano
/
Antibacterianos
Límite:
Adulto
/
Femenino
/
Humanos
País/Región como asunto:
Europa
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2014
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Rumanía
Institución/País de afiliación:
Development for Microbiology and Immunology+RO
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