Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease
Mem. Inst. Oswaldo Cruz
;
109(2): 174-181, abr. 2014. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-705826
ABSTRACT
Chagas disease, caused by Trypanosoma cruzi, represents an endemic among Latin America countries. The participation of free radicals, especially nitric oxide (NO), has been demonstrated in the pathophysiology of seropositive individuals with T. cruzi. In Chagas disease, increased NO contributes to the development of cardiomyopathy and megacolon. Metallothioneins (MTs) are efficient free radicals scavengers of NO in vitro and in vivo. Here, we developed a murine model of the chronic phase of Chagas disease using endemic T. cruzi RyCH1 in BALB/c mice, which were divided into four groups infected non-treated (Inf), infected N-monomethyl-L-arginine treated (Inf L-NAME), non-infected L-NAME treated and non-infected vehicle-treated. We determined blood parasitaemia and NO levels, the extent of parasite nests in tissues and liver MT-I expression levels. It was observed that NO levels were increasing in Inf mice in a time-dependent manner. Inf L-NAME mice had fewer T. cruzi nests in cardiac and skeletal muscle with decreased blood NO levels at day 135 post infection. This affect was negatively correlated with an increase of MT-I expression (r = -0.8462, p < 0.0001). In conclusion, we determined that in Chagas disease, an unknown inhibitory mechanism reduces MT-I expression, allowing augmented NO levels.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Enfermedad de Chagas
/
Metalotioneína
/
Óxido Nítrico
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2014
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
México
Institución/País de afiliación:
Benemérita Universidad Autónoma de Puebla/MX
Similares
MEDLINE
...
LILACS
LIS