Evaluation of polymerase chain reaction (PCR) for identifying Mycobacterium bovis isolates from the modified Middlebrook 7H11 agar thin layer technique
Rev. Inst. Adolfo Lutz
;
71(4): 753-756, out.-dez. 2012. tab
Artículo
en Inglés
| LILACS, SES-SP, SESSP-CTDPROD, SES-SP, SESSP-ACVSES, SESSP-IALPROD, SES-SP, SESSP-IALACERVO
| ID: lil-706148
ABSTRACT
The associated use of the modified Middlebrook 7H11 agar thin layer technique and the Polymerase Chain Reaction (PCR) assay enabled to perform the early identification of microcolonies of Mycobacterium bovis from 12th to 25th day of culture. In order to reduce the time for performing the Mycobacterium bovis identification, the combined use of these two techniques was evaluated by analyzing the microcolonies of mycobacteria at the 8th day after culturing. Until the last day of analysis, all of the PCR-positive samples already showed the microcolonies. Therefore, the early diagnosis of bovine tuberculosis is feasible, without an apparent macroscopic colonies growth.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Tuberculosis
/
Reacción en Cadena de la Polimerasa
/
Técnicas y Procedimientos Diagnósticos
/
Agar
/
Mycobacterium bovis
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Inglés
Revista:
Rev. Inst. Adolfo Lutz
Año:
2012
Tipo del documento:
Artículo
Institución/País de afiliación:
Agência Paulista de Tecnologia dos Agronegócios/BR
/
Universidade de São Paulo/BR
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