Caries-free subjects have high levels of urease and arginine deiminase activity
J. appl. oral sci
;
22(3): 235-240, May-Jun/2014. tab
Artículo
en Inglés
| LILACS, BBO
| ID: lil-711711
ABSTRACT
Objectives:
This study investigated the relationship between urease and arginine deiminase system (ADS) activities and dental caries through a cross-sectional study. Material andMethods:
Urease and ADS activities were measured in saliva and plaque samples from 10 caries-free subjects and 13 caries-active. Urease activity was obtained from the ammonia produced by incubation of plaque and saliva samples in urea. ADS activity was obtained from the ammonia generated by the arginine-HCl and Tris-maleate buffer. Specific activity was defined as micromoles of ammonia per minute per milligram of protein. Shapiro-Wilk statistical test was used to analyze the distribution of the data, and Mann-Whitney test was used to determine the significance of the data.Results:
The specific urease activity in saliva and plaque was significantly higher in individuals with low DMFT scores. ADS activity in saliva (6.050 vs 1.350, p=0.0154) and plaque (8.830 vs 1.210, p=0.025) was also higher in individuals with low DMFT scores.Conclusions:
Caries-free subjects had a higher ammonia generation activity by urease and arginine deiminase system for both saliva and plaque samples than low caries-active subjects. High levels of alkali production in oral environment were related to caries-free subjects. .
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Saliva
/
Ureasa
/
Caries Dental
/
Placa Dental
/
Hidrolasas
Tipo de estudio:
Estudio de etiología
/
Estudio observacional
/
Estudio de prevalencia
/
Factores de riesgo
Límite:
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
J. appl. oral sci
Asunto de la revista:
Odontología
Año:
2014
Tipo del documento:
Artículo
País de afiliación:
Chile
Institución/País de afiliación:
University of Chile/CL
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