Growth kinetics, effect of carbon substrate in biosynthesis of mcl-PHA by Pseudomonas putida Bet001
Braz. j. microbiol
;
45(2): 427-438, Apr.-June 2014. ilus, graf, tab
Artículo
en Inglés
| LILACS
| ID: lil-723098
ABSTRACT
Growth associated biosynthesis of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) in Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. Models with substrate inhibition terms described well the kinetics of its growth. Selected fatty acids (C80 to C181) and ammonium were used as carbon and nitrogen sources during growth and PHA biosynthesis, resulting in PHA accumulation of about 50 to 69% (w/w) and PHA yields ranging from 10.12 g L-1 to 15.45 g L-1, respectively. The monomer composition of the PHA ranges from C4 to C14, and was strongly influenced by the type of carbon substrate fed. Interestingly, an odd carbon chain length (C7) monomer was also detected when C181 was fed. Polymer showed melting temperature (Tm) of 42.0 (± 0.2) °C, glass transition temperature (Tg) of -1.0 (± 0.2) °C and endothermic melting enthalpy of fusion (ΔHf) of 110.3 (± 0.1) J g-1. The molecular weight (Mw) range of the polymer was relatively narrow between 55 to 77 kDa.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Carbono
/
Pseudomonas putida
/
Polihidroxialcanoatos
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2014
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Malasia
Institución/País de afiliación:
University of Malaya/MY
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