Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis
J. venom. anim. toxins incl. trop. dis
; 2004/02/2014.
Article
en En
| LILACS
| ID: lil-724683
Biblioteca responsable:
BR68.1
ABSTRACT
Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect the sensitivity of PCR assays, including DNA yield and purity, an optimal extraction method is pivotal. Losses of a parasites DNA during extraction may significantly impair its detection by PCR and lead to false-negative results. This study proposes a triplex PCR assay targeting the parasites DNA, an external control (pUC18) and an internal control (G3PD) for accurate diagnosis of leishmaniasis.
Palabras clave
Texto completo:
1
Índice:
LILACS
Asunto principal:
Leishmaniasis
/
Reacción en Cadena de la Polimerasa
/
Diagnóstico
/
Reacciones Falso Negativas
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
J. venom. anim. toxins incl. trop. dis
Asunto de la revista:
TOXICOLOGIA
Año:
2014
Tipo del documento:
Article