The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation
Acta cir. bras
;
29(supl.3): 28-32, 2014. graf
Artículo
en Inglés
| LILACS
| ID: lil-726240
ABSTRACT
PURPOSE:
To evaluate the NAC effects on liver hypothermic preservation at different time intervals.METHODS:
For this, we used livers of male Wistar rats weighing between 250 and 300g, undergoing preservation in Ringer solution at 4°C for up to 24 hours. Tissue samples were obtained at four moments of preservation for histological examination by hematoxylin and eosin staining T0 = beginning of preservation, T12 = 12 hours, T18 = 18 hours and T24 = 24 hours. Will be analyzed vacuolation, hepatic apoptosis by optical microscopy and parenchymal.RESULTS:
The results showed a progressive increase in hepatic injury in both groups and showed that NAC was effective at T0. The parenchyma preservation was better in the NAC group and no difference when vacuolization of the cells.CONCLUSION:
Hypothermic preservation, over time, causes changes in the hepatic parenchyma with increased apoptosis, loss of architecture, vacuolization, culminating in severe injury. The administration of N-acetylcysteine protects against preservation liver injury. .
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Preservación de Órganos
/
Acetilcisteína
/
Criopreservación
/
Depuradores de Radicales Libres
/
Hígado
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Acta cir. bras
Asunto de la revista:
Cirugía General
/
Procedimentos Cir£rgicos Operat¢rios
Año:
2014
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
University City of São Paulo/BR
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