Construction of three new Gateway® expression plasmids for Trypanosoma cruzi
Mem. Inst. Oswaldo Cruz
;
109(8): 1081-1085, 12/2014. graf
Artículo
en Inglés
| LILACS
| ID: lil-732602
ABSTRACT
We present here three expression plasmids for Trypanosoma cruzi adapted to the Gateway® recombination cloning system. Two of these plasmids were designed to express trypanosomal proteins fused to a double tag for tandem affinity purification (TAPtag). The TAPtag and Gateway® cassette were introduced into an episomal (pTEX) and an integrative (pTREX) plasmid. Both plasmids were assayed by introducing green fluorescent protein (GFP) by recombination and the integrity of the double-tagged protein was determined by western blotting and immunofluorescence microscopy. The third Gateway adapted vector assayed was the inducible pTcINDEX. When tested with GFP, pTcINDEX-GW showed a good response to tetracycline, being less leaky than its precursor (pTcINDEX).
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Plásmidos
/
Trypanosoma cruzi
/
Mapeo Restrictivo
/
Expresión Génica
/
Vectores Genéticos
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2014
Tipo del documento:
Artículo
País de afiliación:
Argentina
Institución/País de afiliación:
Universidad Nacional de Rosario/AR
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