Rapid detection of Salmonella Typhi by loop-mediated isothermal amplification (LAMP) method
Braz. j. microbiol
;
45(4): 1385-1391, Oct.-Dec. 2014. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-741291
ABSTRACT
An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity in detecting the presence of Salmonella Typhi (S. Typhi) isolates from Kelantan, Malaysia. Three sets of primers consisting of two outer and 4 inner were designed based on locus STBHUCCB_38510 of chaperone PapD of S. Typhi genes. The reaction was optimised using genomic DNA of S. Typhi ATCC7251 as the template. The products were visualised directly by colour changes of the reaction. Positive results were indicated by green fluorescence and negative by orange colour. The test was further evaluated for specificity, sensitivity and application on field samples. The results were compared with those obtained by gold standard culture method and Polymerase Chain Reaction (PCR). This method was highly specific and -10 times more sensitive in detecting S. Typhi compared to the optimised conventional polymerase chain reaction (PCR) method.
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Salmonella typhi
/
Fiebre Tifoidea
/
Técnicas Bacteriológicas
/
Técnicas de Amplificación de Ácido Nucleico
/
Técnicas de Diagnóstico Molecular
Tipo de estudio:
Estudio diagnóstico
/
Estudios de evaluación
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2014
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Malasia
Institución/País de afiliación:
Institute for Research in Molecular Medicine/MY
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