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Adherence and virulence genes of Escherichia coli from children diarrhoea in the Brazilian Amazon
Benevides-Matos, Najla; Pieri, Fabio A.; Penatti, Marilene; Orlandi, Patrícia P..
  • Benevides-Matos, Najla; Fundação Oswaldo Cruz. Instituto de Pesquisas em Patologias Tropicais. Porto Velho. BR
  • Pieri, Fabio A.; Fundação Oswaldo Cruz. Instituto de Pesquisas em Patologias Tropicais. Porto Velho. BR
  • Penatti, Marilene; Fundação Oswaldo Cruz. Instituto de Pesquisas em Patologias Tropicais. Porto Velho. BR
  • Orlandi, Patrícia P.; Fundação Oswaldo Cruz. Instituto de Pesquisas em Patologias Tropicais. Porto Velho. BR
Braz. j. microbiol ; 46(1): 131-137, 05/2015. tab, graf
Artículo en Inglés | LILACS | ID: lil-748251
ABSTRACT
The bacterial pathogen most commonly associated with endemic forms of childhood diarrhoea is Escherichia coli. Studies of epidemiological characteristics of HEp-2 cell-adherent E. coli in diarrhoeal disease are required, particularly in developing countries. The aim of this study was evaluate the presence and significance of adherent Escherichia coli from diarrhoeal disease in children. The prevalence of LA, AA, and DA adherence patterns were determined in HEp-2 cells, the presence of virulence genes and the presence of the O serogroups in samples obtained from 470 children with acute diarrhoea and 407 controls in Porto Velho, Rondônia, Brazil. E. coli isolates were identified by PCR specific for groups of adherent E. coli. Out of 1,156 isolates obtained, 128 (11.0%) were positive for eae genes corresponding to EPEC, however only 38 (29.6%) of these amplified bfpA gene. EAEC were isolated from 164 (14.1%) samples; of those 41(25%), 32 (19%) and 16 (9.7%) amplified eagg, aggA or aafA genes, respectively and aggA was significantly associated with diarrhoea (P = 0.00006). DAEC identified by their adhesion pattern and there were few isolates. In conclusion, EAEC was the main cause of diarrhoea in children, especially when the aggA gene was present, followed by EPEC and with a negligible presence of DAEC.
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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Factores de Virulencia / Diarrea / Escherichia coli / Infecciones por Escherichia coli / Escherichia coli Enteropatógena Tipo de estudio: Estudio pronóstico / Factores de riesgo Límite: Niño / Child, preschool / Humanos / Lactante / Recién Nacido País/Región como asunto: America del Sur / Brasil Idioma: Inglés Revista: Braz. j. microbiol Asunto de la revista: Microbiologia Año: 2015 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Fundação Oswaldo Cruz/BR

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Factores de Virulencia / Diarrea / Escherichia coli / Infecciones por Escherichia coli / Escherichia coli Enteropatógena Tipo de estudio: Estudio pronóstico / Factores de riesgo Límite: Niño / Child, preschool / Humanos / Lactante / Recién Nacido País/Región como asunto: America del Sur / Brasil Idioma: Inglés Revista: Braz. j. microbiol Asunto de la revista: Microbiologia Año: 2015 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Fundação Oswaldo Cruz/BR