A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivax and Plasmodium falciparum infection in field-collected anophelines
Mem. Inst. Oswaldo Cruz
;
110(4): 573-576, 09/06/2015. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-748860
ABSTRACT
We describe a simple method for detection of Plasmodium vivax and Plasmodium falciparum infection in anophelines using a triplex TaqMan real-time polymerase chain reaction (PCR) assay (18S rRNA). We tested the assay on Anopheles darlingi and Anopheles stephensi colony mosquitoes fed with Plasmodium-infected blood meals and in duplicate on field collected An. darlingi. We compared the real-time PCR results of colony-infected and field collected An. darlingi, separately, to a conventional PCR method. We determined that a cytochrome b-PCR method was only 3.33% as sensitive and 93.38% as specific as our real-time PCR assay with field-collected samples. We demonstrate that this assay is sensitive, specific and reproducible.
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Asunto principal:
Plasmodium falciparum
/
Plasmodium vivax
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Insectos Vectores
/
Anopheles
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2015
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Estados Unidos
Institución/País de afiliación:
New York State Department of Health/US
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