Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections
Mem. Inst. Oswaldo Cruz
;
110(4): 577-579, 09/06/2015. graf
Artículo
en Inglés
| LILACS
| ID: lil-748866
ABSTRACT
An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Inmunoglobulinas
/
Virus de la Hepatitis A
/
Hepatitis A
/
Hígado
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2015
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Instituto Oswaldo Cruz/BR
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