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Mobilization and collection of CD34+ cells for autologous transplantation of peripheral blood hematopoietic progenitor cells in children: analysis of two different granulocyte-colony stimulating factor doses
Eid, Kátia Aparecida de Brito; Miranda, Eliana Cristina Martins; Aguiar, Simone dos Santos.
  • Eid, Kátia Aparecida de Brito; Universidade Estadual de Campinas. BR
  • Miranda, Eliana Cristina Martins; Universidade Estadual de Campinas. BR
  • Aguiar, Simone dos Santos; Universidade Estadual de Campinas. BR
Rev. bras. hematol. hemoter ; 37(3): 160-166, May-Jun/2015. tab, graf
Artículo en Inglés | LILACS | ID: lil-752536
ABSTRACT
The use of peripheral hematopoietic progenitor cells (HPCs) is the cell choice in autologous transplantation. The classic dose of granulocyte-colony stimulating factor (G- CSF) for mobilization is a single daily dose of 10 µg/kg of patient body weight. There is a theory that higher doses of granulocyte-colony stimulating factor applied twice daily could increase the number of CD34+ cells collected in fewer leukapheresis procedures.

Objective:

The aim of this study was to compare a fractionated dose of 15 µg G-CSF/kg of body weight and the conventional dose of granulocyte-colony stimulating factor in respect to the number of leukapheresis procedures required to achieve a minimum collection of 3 × 106 CD34+ cells/kg body weight.

Methods:

Patients were divided into two groups Group 10 - patients who received a single daily dose of 10 µg G-CSF/kg body weight and Group 15 - patients who received a fractioned dose of 15 µg G-CSF/kg body weight daily. The leukapheresis procedure was carried out in an automated cell separator. The autologous transplantation was carried out when a minimum number of 3 × 106 CD34+ cells/kg body weight was achieved.

Results:

Group 10 comprised 39 patients and Group 15 comprised 26 patients. A total of 146 apheresis procedures were performed 110 (75.3%) for Group 10 and 36 (24.7%) for Group 15. For Group 10, a median of three (range 1-7) leukapheresis procedures and a mean of 8.89 × 106 CD34+ cells/kg body weight (±9.59) were collected whereas for Group 15 the corresponding values were one (range 1-3) and 5.29 × 106 cells/kg body weight (±4.95). A statistically significant difference was found in relation to the number of apheresis procedures (p-value <0.0001).

Conclusions:

To collect a minimum target of 3 × 106 CD34+ cells/kg body weight, the administration of a fractionated dose of 15 µg G-CSF/kg body weight significantly decreased the number of leukapheresis procedures performed.
Asunto(s)


Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Trasplante Autólogo / Factor Estimulante de Colonias de Granulocitos / Leucaféresis / Trasplante de Células Madre Hematopoyéticas / Movilización de Célula Madre Hematopoyética / Trasplante de Células Madre de Sangre Periférica Límite: Adolescente / Niño / Child, preschool / Femenino / Humanos / Lactante / Masculino / Recién Nacido Idioma: Inglés Revista: Rev. bras. hematol. hemoter Asunto de la revista: Hematología Año: 2015 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Universidade Estadual de Campinas/BR

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Trasplante Autólogo / Factor Estimulante de Colonias de Granulocitos / Leucaféresis / Trasplante de Células Madre Hematopoyéticas / Movilización de Célula Madre Hematopoyética / Trasplante de Células Madre de Sangre Periférica Límite: Adolescente / Niño / Child, preschool / Femenino / Humanos / Lactante / Masculino / Recién Nacido Idioma: Inglés Revista: Rev. bras. hematol. hemoter Asunto de la revista: Hematología Año: 2015 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Universidade Estadual de Campinas/BR