Effect of remote ischemic preconditioning in the expression of IL-6 and IL-10 in a rat model of liver ischemia-reperfusion injury
Acta cir. bras
;
30(7): 452-460, 07/2015. graf
Artículo
en Inglés
| LILACS
| ID: lil-754977
ABSTRACT
PURPOSE:
To study the effect of remote ischemic preconditioning (RIPC) in ischemia-reperfusion (I/R) liver injury and in the expression of IL-6 and IL-10 in a rat model.METHODS:
Thirty-six male rats were divided in three groups Sham; I/R injury, a 45 minutes lobar liver ischemia and reperfusion; and RIPC, six cycles of four minutes of ischemia and four minutes of reperfusion on the right hindlimb followed by a 45 minutes lobar liver ischemia and reperfusion. Tissue and blood samples were collected after 1h and 3h of reperfusion for histopathological study, plasma cytokines and alanine aminotransferase (ALT) measurement.RESULTS:
The histopathological study demonstrated a significant reduction in liver necrosis in the RIPC group (p<0,001). The ALT levels were also significant lower in the RIPC group (p<0.01). The cytokines assessment showed that IL-6 levels were increased in the RIPC group after 1h of reperfusion, in comparison to the I/R group (p<0.05). Interleukin-10 levels in RIPC groups did not differ significantly from I/R group.CONCLUSIONS:
Remote ischemic preconditioning is effective in decreasing liver necrosis in a rat model of ischemia-reperfusion. The IL-6 expression is up-regulated and peaked at 60 min of reperfusion. There was no difference in IL-10 expression between the groups. .
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Daño por Reperfusión
/
Precondicionamiento Isquémico
/
Modelos Animales de Enfermedad
/
Hígado
Tipo de estudio:
Estudios de evaluación
/
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Acta cir. bras
Asunto de la revista:
Cirugía General
/
Procedimentos Cir£rgicos Operat¢rios
Año:
2015
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Estadual do Rio de Janeiro/BR
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