Agreement between rapd, api20c aux, chromagar candida and microculture on oral candida identification
Braz. j. oral sci
;
14(2): 149-153, Apr.-June 2015. tab, ilus
Artículo
en Inglés
| LILACS
| ID: lil-755047
ABSTRACT
Aim:
To measure the agreement of methods for identification of Candida species in oral cavity samples, comparing the CHROMagar Candida, microculture, API 20C AUX and RAPD techniques.Methods:
Ninety-one colonies of Candida were isolated and presumptively identified in CHROMagar Candida, submitted to microculture, API 20C AUX and RAPD techniques. After this, agreement among methods using Kappa test was performed.Results:
Agreement rates between RAPD and CHROMagar Candida, showed significant accuracy for C. albicans, C. tropicalis, C. dubliniensis and C. krusei (Kappa 0.760, 0.640, 0.416 and 0.360, respectively, p<0.05). Comparing RAPD results with microculture, the highest agreement was for C. albicans (Kappa 0.851 - p<0.05) but no significant agreement for C. lusitaniae, C. krusei and C. guilliermondii was obtained (p>0.05). The agreement was significant for all identified species when RAPD (OPE-18) and API 20C AUX (p<0.05) were used. Critical levels of agreement between RAPD and microculture were observed when C. lusitaniae, C. krusei and C. guilliermondii were identified.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Candida
/
Métodos
/
Boca
Tipo de estudio:
Estudio diagnóstico
Límite:
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Braz. j. oral sci
Asunto de la revista:
Odontología
Año:
2015
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Estadual de Montes Claros/BR
/
Universidade Federal da Paraíba/BR
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