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Assessment of the pathogenicity of cell-culture-adapted Newcastle disease virus strain Komarov
Visnuvinayagam, Sivam; K, Thangavel; N, Lalitha; S, Malmarugan; Sukumar, Kuppannan.
  • Visnuvinayagam, Sivam; Central Institute of Fisheries Technology. Mumbai Research Centre. Mumbai. IN
  • K, Thangavel; Central Institute of Fisheries Technology. Mumbai Research Centre. Mumbai. IN
  • N, Lalitha; Central Institute of Fisheries Technology. Mumbai Research Centre. Mumbai. IN
  • S, Malmarugan; Central Institute of Fisheries Technology. Mumbai Research Centre. Mumbai. IN
  • Sukumar, Kuppannan; Central Institute of Fisheries Technology. Mumbai Research Centre. Mumbai. IN
Braz. j. microbiol ; 46(3): 861-865, July-Sept. 2015. tab, ilus
Artículo en Inglés | LILACS | ID: lil-755806
ABSTRACT

Newcastle disease vaccines hitherto in vogue are produced from embryonated chicken eggs. Egg-adapted mesogenic vaccines possess several drawbacks such as paralysis and mortality in 2-week-old chicks and reduced egg production in the egg-laying flock. Owing to these possible drawbacks, we attempted to reduce the vaccine virulence for safe vaccination by adapting the virus in a chicken embryo fibroblast cell culture (CEFCC) system. Eighteen passages were carried out by CEFCC, and the pathogenicity was assessed on the basis of the mean death time, intracerebral pathogenicity index, and intravenous pathogenicity index, at equal passage intervals. Although the reduction in virulence demonstrated with increasing passage levels in CEFCC was encouraging, 20% of the 2-week-old birds showed paralytic symptoms with the virus vaccine from the 18th(final) passage. Thus, a tissue-culture-adapted vaccine would demand a few more passages by CEFCC in order to achieve a complete reduction in virulence for use as a safe and effective vaccine, especially among younger chicks. Moreover, it can be safely administered even to unprimed 8-week-old birds.

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Enfermedades de las Aves de Corral / Virus de la Enfermedad de Newcastle / Vacunas Atenuadas / Vacunas Virales / Pollos Límite: Animales Idioma: Inglés Revista: Braz. j. microbiol Asunto de la revista: Microbiologia Año: 2015 Tipo del documento: Artículo País de afiliación: India Institución/País de afiliación: Central Institute of Fisheries Technology/IN

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Enfermedades de las Aves de Corral / Virus de la Enfermedad de Newcastle / Vacunas Atenuadas / Vacunas Virales / Pollos Límite: Animales Idioma: Inglés Revista: Braz. j. microbiol Asunto de la revista: Microbiologia Año: 2015 Tipo del documento: Artículo País de afiliación: India Institución/País de afiliación: Central Institute of Fisheries Technology/IN