Gene cloning, expression, and characterization of the Bacillus amyloliquefaciens PS35 lipase
Braz. j. microbiol
;
46(4): 1235-1243, Oct.-Dec. 2015. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-769636
ABSTRACT
Abstract Lipases are enzymes of immense industrial relevance, and, therefore, are being intensely investigated. In an attempt to characterize lipases at molecular level from novel sources, a lipase gene from Bacillus amyloliquefaciens PS35 was cloned, heterologously expressed in Escherichia coli DH5α cells and sequenced. It showed up to 98% homology with other lipase sequences in the NCBI database. The recombinant enzyme was then purified from E. coli culture, resulting in a 19.41-fold purification with 9.7% yield. It displayed a preference for long-chain para-nitrophenyl esters, a characteristic that is typical of true lipases. Its optimum pH and temperature were determined to be 8.0 and 40 °C, respectively. The half-lives were 2.0, 1.0 and 0.5 h at 50 °C, 60 °C and 70 °C, respectively. The metal ions K+ and Fe3+ enhanced the enzyme activity. The enzyme displayed substantial residual activity in the presence of various tested chemical modifiers, and interestingly, the organic solvents, such as n-hexane and toluene, also favored the enzyme activity. Thus, this study involves characterization of B. amyloliquefaciens lipase at molecular level. The key outcomes are novelty of the bacterial source and purification of the enzyme with desirable properties for industrial applications.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Características de la Residencia
/
Dieta
/
Planificación Ambiental
/
Abastecimiento de Alimentos
/
Obesidad
Tipo de estudio:
Investigación cualitativa
Límite:
Adulto
/
Femenino
/
Humanos
/
Masculino
País/Región como asunto:
America del Norte
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2015
Tipo del documento:
Artículo
País de afiliación:
India
Institución/País de afiliación:
Kumaraguru College of Technology/IN
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