Analysis for the presence of determinants involved in the transport of mercury across bacterial membrane from polluted water bodies of India
Braz. j. microbiol
;
47(1): 55-62, Jan.-Mar. 2016. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-775121
ABSTRACT
Abstract Mercury, which is ubiquitous and recalcitrant to biodegradation processes, threatens human health by escaping to the environment via various natural and anthropogenic activities. Non-biodegradability of mercury pollutants has necessitated the development and implementation of economic alternatives with promising potential to remove metals from the environment. Enhancement of microbial based remediation strategies through genetic engineering approaches provides one such alternative with a promising future. In this study, bacterial isolates inhabiting polluted sites were screened for tolerance to varying concentrations of mercuric chloride. Following identification, several Pseudomonas and Klebsiella species were found to exhibit the highest tolerance to both organic and inorganic mercury. Screened bacterial isolates were examined for their genetic make-up in terms of the presence of genes (merP and merT) involved in the transport of mercury across the membrane either alone or in combination to deal with the toxic mercury. Gene sequence analysis revealed that the merP gene showed 86–99% homology, while the merT gene showed >98% homology with previously reported sequences. By exploring the genes involved in imparting metal resistance to bacteria, this study will serve to highlight the credentials that are particularly advantageous for their practical application to remediation of mercury from the environment.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Proteínas de Transporte de Membrana
/
Pseudomonas
/
Contaminantes Químicos del Agua
/
Klebsiella
/
Mercurio
Límite:
Humanos
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2016
Tipo del documento:
Artículo
País de afiliación:
Corea del Sur
Institución/País de afiliación:
Yeungnam University/KR
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