Your browser doesn't support javascript.
loading
Three new shuttle vectors for heterologous expression in Zymomonas mobilis
Cao, Qinghua; Li, Tao; Shao, Huanhuan; Tan, Xuemei; Zhang, Yizheng.
  • Cao, Qinghua; Sichuan University. Sichuan Key Laboratory of Molecular Biology and Biotechnology. Ministry of Education. College of Life Sciences, Key Laboratory of Bio-resources and Eco-environment. Sichuan. CN
  • Li, Tao; Sichuan University. Sichuan Key Laboratory of Molecular Biology and Biotechnology. Ministry of Education. College of Life Sciences, Key Laboratory of Bio-resources and Eco-environment. Sichuan. CN
  • Shao, Huanhuan; Sichuan University. Sichuan Key Laboratory of Molecular Biology and Biotechnology. Ministry of Education. College of Life Sciences, Key Laboratory of Bio-resources and Eco-environment. Sichuan. CN
  • Tan, Xuemei; Sichuan University. Sichuan Key Laboratory of Molecular Biology and Biotechnology. Ministry of Education. College of Life Sciences, Key Laboratory of Bio-resources and Eco-environment. Sichuan. CN
  • Zhang, Yizheng; Sichuan University. Sichuan Key Laboratory of Molecular Biology and Biotechnology. Ministry of Education. College of Life Sciences, Key Laboratory of Bio-resources and Eco-environment. Sichuan. CN
Electron. j. biotechnol ; 19(1): 33-40, Jan. 2016. ilus
Artículo en Inglés | LILACS | ID: lil-781168
ABSTRACT

Background:

Zymomonas mobilis, as a novel platform for bio-ethanol production, has been attracted more attention and it is very important to construct vectors for the efficient expression of foreign genes in this bacterium.

Results:

Three shuttle vectors ( pSUZM 1, pSUZM2 and pSUZM3 ) were first constructed with the origins of replication from the chromosome and two native plasmids (pZZM401 and pZZM402) of Z. mobilis ZM4, respectively. The three shuttle vectors were stable in Z. mobilis ZM4 and have 3,32 and 27 copies, respectively. The promoter Ppdc (a), from the pyruvate decarboxylase gene, was clonedinto the shuttle vectors, generatingthe expressionvectors pSUZM1(2, 3)a. The codon-optimized glucoamylase gene from Aspergillus awamori combined with the signal peptide sequence from the alkaline phosphatase gene of Z. mobilis was cloned into pSUZM1(2, 3)a, resulting in the plasmids pSUZM1a-GA, pSUZM2a-GA and pSUZM3a-GA, respectively. After transforming these plasmids into Z. mobilis ZM4, the host was endowed with glucoamylase activity for starch hydrolysis. Both pSUZM2a-GA and pSUZM3a-GA were more efficientatproducingglucoamylase thanpSUZM1a-GA.

Conclusions:

These results indicated that these expression vectors are useful tools for gene expression in Z. mobilis and this could provide a solid foundation for further studies of heterologous gene expression in Z. mobilis.
Asunto(s)


Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Expresión Génica / Zymomonas / Vectores Genéticos Idioma: Inglés Revista: Electron. j. biotechnol Asunto de la revista: Biotecnologia Año: 2016 Tipo del documento: Artículo País de afiliación: China Institución/País de afiliación: Sichuan University/CN

Similares

MEDLINE

...
LILACS

LIS


Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Expresión Génica / Zymomonas / Vectores Genéticos Idioma: Inglés Revista: Electron. j. biotechnol Asunto de la revista: Biotecnologia Año: 2016 Tipo del documento: Artículo País de afiliación: China Institución/País de afiliación: Sichuan University/CN