Bioconversion of mixed free fatty acids to poly-3-hydroxyalkanoates by Pseudomonas putida BET001 and modeling of its fermentation in shake flasks
Electron. j. biotechnol
;
19(1): 50-55, Jan. 2016. ilus
Artículo
en Inglés
| LILACS
| ID: lil-781170
ABSTRACT
Background:
The paper reports on the utilization of palm kernel oil (PKO) as a low cost renewable substrate for medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) production by Pseudomonas putida BET001. Investigation on the effects of selected key variables on growth, mixed free fatty acids consumption and mcl-PHA production by the bacterial culture in the shaken flask system were carried out along with its kinetic modeling.Results:
The biomass production, fatty acids consumption and mcl-PHA production were found favorable when the strain was cultured in mineral medium at pH 6-7,28°C, aeration surface-to-volume ratio of 0.4 x 10(6) m-1, 250 rpm agitation rate for 48 h. Mcl-PHA production by this strain showed mixed growth and non-growth associated components as described by Luedeking-Piret kinetic model.Conclusion:
The findings of this study provided add to the literature on key variables in for achieving good microbial growth and mcl-PHA production in shake flasks culture. In addition, suitable kinetic model to describe cultivation in this system was also presented.
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Biodegradación Ambiental
/
Biopolímeros
/
Pseudomonas putida
/
Ácidos Grasos
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Electron. j. biotechnol
Asunto de la revista:
Biotecnologia
Año:
2016
Tipo del documento:
Artículo
País de afiliación:
Malasia
Institución/País de afiliación:
University of Malaya/MY
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