Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
Mem. Inst. Oswaldo Cruz
;
111(5): 349-354, May 2016. graf
Artículo
en Inglés
| LILACS
| ID: lil-782047
ABSTRACT
During its life cycle Leishmania spp. face several stress conditions that can cause DNA damages. Base Excision Repair plays an important role in DNA maintenance and it is one of the most conserved mechanisms in all living organisms. DNA repair in trypanosomatids has been reported only for Old World Leishmania species. Here the AP endonuclease from Leishmania (L.) amazonensis was cloned, expressed in Escherichia coli mutants defective on the DNA repair machinery, that were submitted to different stress conditions, showing ability to survive in comparison to the triple null mutant parental strain BW535. Phylogenetic and multiple sequence analyses also confirmed that LAMAP belongs to the AP endonuclease class of proteins.
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Leishmania braziliensis
/
Daño del ADN
/
ADN-(Sitio Apurínico o Apirimidínico) Liasa
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Reparación del ADN
/
Escherichia coli
/
Mutación
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2016
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade do Estado do Rio de Janeiro/BR
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