The prevalence of aminoglycoside-modifying enzyme and virulence genes among enterococci with high-level aminoglycoside resistance in Inner Mongolia, China
Braz. j. microbiol
;
47(3): 691-696, July-Sept. 2016. tab
Artículo
en Inglés
| LILACS
| ID: lil-788958
ABSTRACT
ABSTRACT This study highlights the prevalence of aminoglycoside-modifying enzyme genes and virulence determinants among clinical enterococci with high-level aminoglycoside resistance in Inner Mongolia, China. Screening for high-level aminoglycoside resistance against 117 enterococcal clinical isolates was performed using the agar-screening method. Out of the 117 enterococcal isolates, 46 were selected for further detection and determination of the distribution of aminoglycoside-modifying enzyme-encoding genes and virulence determinants using polymerase chain reaction -based methods. Enterococcus faecium and Enterococcus faecalis were identified as the species of greatest clinical importance. The aac(6')-Ie-aph(2")-Ia and ant(6')-Ia genes were found to be the most common aminoglycoside-modifying enzyme genes among high-level gentamicin resistance and high-level streptomycin resistance isolates, respectively. Moreover, gelE was the most common virulence gene among high-level aminoglycoside resistance isolates. Compared to Enterococcus faecium, Enterococcus faecalis harbored multiple virulence determinants. The results further indicated no correlation between aminoglycoside-modifying enzyme gene profiles and the distribution of virulence genes among the enterococcal isolates with high-level gentamicin resistance or high-level streptomycin resistance evaluated in our study.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Regulación Bacteriana de la Expresión Génica
/
Regulación Enzimológica de la Expresión Génica
/
Enterococcus
/
Farmacorresistencia Bacteriana
/
Aminoglicósidos
Tipo de estudio:
Estudio de prevalencia
/
Factores de riesgo
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2016
Tipo del documento:
Artículo
País de afiliación:
China
Institución/País de afiliación:
Baotou Medical College/CN
Similares
MEDLINE
...
LILACS
LIS