Detection of Leishmania (Viannia) braziliensis by PCR in samples collected by scraping the lesion edges from patients of an endemic area
Rev. Inst. Adolfo Lutz (Online)
;
74(4): 437-441, out.-dez.2015. ilus, tab
Artículo
en Inglés
| LILACS, SES-SP
| ID: lil-797169
ABSTRACT
American cutaneous leishmaniasis (ACL) is an infectious disease caused by Leishmania. Their diagnosis is performed in samples collected from the lesion biopsies, which has to be performedby physicians. For simplifying the sample collection, this study proposes a minimally invasive procedure, by scraping the lesion edges. Laboratory diagnosis by PCR was performed and compared with the microscopic examination, by analyzing 28 samples collected from patients with suspicion of ACL. Sample collected from the lesion edge with a sterile toothpick was divided into two aliquots. One aliquot was analyzed under direct microscopy, and the second by PCR, by using two primer pairs (one for Leishmania genus and other for L. (V.) braziliensis). Of 28 samples, 27 (96.43 %) showed concordant results in both methodologies (eight positive and 20 negative). The PCR methodologyis an invaluable tool (i) to determine the Leishmania species; (ii) to provide an alternative procedure of sample collection, when an authorized professional is not available in the respective health service;and (iii) to propose a minimally invasive procedure for collecting biological material...
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Leishmania braziliensis
/
Reacción en Cadena de la Polimerasa
/
Leishmaniasis Cutánea
Tipo de estudio:
Estudio diagnóstico
Límite:
Femenino
/
Humanos
/
Masculino
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Rev. Inst. Adolfo Lutz (Online)
Año:
2015
Tipo del documento:
Artículo
Institución/País de afiliación:
Instituto Adolfo Lutz/BR
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