Buffering action of acetate on hydrogen production by Ethanoligenens harbinense B49
Electron. j. biotechnol
;
19(5): 2-11, Sept. 2016. ilus
Artículo
en Inglés
| LILACS
| ID: lil-797331
ABSTRACT
The buffering effect of acetate on hydrogen production during glucose fermentation by Ethanoligenens harbinense B49 was investigated compared to phosphate, a widely used fermentative hydrogen production buffer. Specific concentrations of sodium acetate or phosphate were added to batch cultures, and the effects on hydrogen production were comparatively analyzed using a modified Gompertz model. Adding 50 mM acetate or phosphate suppressed the hydrogen production peak and slightly extended the lag phase. However, the overall hydrogen yields were 113.5 and 108.5 mmol/L, respectively, and the final pH was effectively controlled. Acetate buffered against hydrogen production more effectively than did phosphate, promoting cell growth and preventing decreased pH. At buffer concentrations 100-250 mM, the maximum hydrogen production was barely suppressed, and the lag phase extended past 7 h. Therefore, although acetate inhibits hydrogen production, using acetate as a buffer (like phosphate) effectively prevented pH drops and increased substrate consumption, enhancing hydrogen production.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Bacterias Grampositivas
/
Hidrógeno
/
Acetatos
Idioma:
Inglés
Revista:
Electron. j. biotechnol
Asunto de la revista:
Biotecnologia
Año:
2016
Tipo del documento:
Artículo
País de afiliación:
China
Institución/País de afiliación:
Inner Mongolia University/CN
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