Ingeniería genética en hongos filamentosos: clonamiento del gen de invertasa de neurospora crassa / Genetic engineering in filamentous fungi: cloning of the invertase gene from Neurospora crassa
Arch. biol. med. exp
; 23(2): 165-72, oct. 1990. tab
Article
en Es
| LILACS
| ID: lil-96788
Biblioteca responsable:
CL1.1
RESUMO
The invertase wild type gene of N. crassa was cloned into the YRp7 yeast vector. This recombinant plasmid was selected by functional complementation of an invertaseless mutant strain of S. cerevisiae. The isolated recombinant plasmid (named pNC2) carries a 7.6 Kb BamHI DNA fragment from N. crassa. The cloned DNA hydbridized with the N. crassa genomic DNA and transformed an invertase mutant of N. crassa Inv- to Inv+. Transformation of N. crassa Inv- to Inv+ seems to take at least two different integration events. One of them involves an integration closely linked to inv locus, and the other one apparently involves as integration of cloned DNA at a genomic site different that the inv locus
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Índice:
LILACS
Asunto principal:
Clonación Molecular
/
Genes Fúngicos
/
Glicósido Hidrolasas
/
Neurospora crassa
Idioma:
Es
Revista:
Arch. biol. med. exp
Asunto de la revista:
BIOLOGIA
Año:
1990
Tipo del documento:
Article