Immunological studies with the venom of the scorpion Tityus serrulatus
Braz. j. med. biol. res
; 24(2): 171-80, 1991. ilus
Article
en En
| LILACS
| ID: lil-99453
Biblioteca responsable:
BR26.1
ABSTRACT
We describe a "sandwich" enzyme-linkedimmunosorbent assay (ELISA) sensitive to quantities of scorpion (Tityus serrulatus) venom (TsV) in the range of 1-3 ng?ml sample. Cross-reactivity with the venom from the rattlesnake Crotalus durissus terrificus and with venoms from several snakes of the Bothrops genus was detected only at concentrations higher than 1 *g/ml sample. A conventional ELISA is also described for the detection of antibodies against TsV. Analysis by Western Blot (WB) demonstrated a 25-kDa protein band common to TsV and to the venoms of Bothrops moojeni, B. jararacussu and B. jararaca. Venom from C. d. terrificus exhibited WB cross-reactive bands of 16 and 25 kDa with TsV
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Índice:
LILACS
Asunto principal:
Venenos de Escorpión
/
Inmunoglobulina G
/
Inmunización
Límite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
Asunto de la revista:
BIOLOGIA
/
MEDICINA
Año:
1991
Tipo del documento:
Article