Protective effect of endothelin antagonist (TAK-044) on neuronal cell viability in in vitro oxygen-glucose deprivation model of stroke.
Indian J Physiol Pharmacol
;
2006 Apr-Jun; 50(2): 157-62
Artículo
en Inglés
| IMSEAR
| ID: sea-107384
ABSTRACT
The present study was carried to investigate the effect of endothelin antagonist (TAK-044) in an in vitro model of stroke using primary neuronal culture. Hypoxia in neuronal culture was induced for 3 h using oxygen glucose deprivation (OGD) model, thereafter cells were reperfused. In separate group cultures were incubated with graded concentrations of TAK-044 (0.01, 0.1 and 1 microg/microl) for different time duration i.e. 6, 12 and 24 hours after reperfusion. Percent cell viability was assessed 24 h after reperfusion using MTT assay. It was observed that percent cell viability was reduced to 13.7 +/- 0.4% in the cells under 3 h hypoxic condition as compared to the cells under normal condition (100%). TAK-044 at the concentrations of 0.1 and 1 microg/microl, but not at 0.01 microg/microl showed significant (P<0.01) improvement in the % cell viability as compared to the cells in hypoxic condition. Percent cell viability at the concentration of 0.1 and 1 microg/microl for 24 h time duration after reperfusion were 54.8 +/- 3.2% and 75.4 +/- 1.8% respectively as compared to the cells under hypoxic condition (13.7 +/- 0.4%). The results demonstrate the neuroprotective effect of TAK-044 against neuronal damage caused by hypoxia induced in neuronal culture.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Péptidos Cíclicos
/
Ratas
/
Hipoxia de la Célula
/
Supervivencia Celular
/
Células Cultivadas
/
Isquemia Encefálica
/
Fármacos Neuroprotectores
/
Accidente Cerebrovascular
/
Relación Dosis-Respuesta a Droga
/
Glucosa
Idioma:
Inglés
Revista:
Indian J Physiol Pharmacol
Año:
2006
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS