An allele-specific polymerase chain reaction assay for the differentiation of members of the Anopheles culicifacies complex.
J Biosci
;
2004 Sep; 29(3): 275-80
Artículo
en Inglés
| IMSEAR
| ID: sea-110613
ABSTRACT
Anopheles culicifacies, the principal vector of malaria in India, is a complex of five cryptic species which are morphologically indistinguishable at any stage of life. In view of the practical difficulties associated with classical cytotaxonomic method for the identification of members of the complex, an allele-specific polymerase chain reaction (ASPCR) assay targeted to the D3 domain of 28S ribosomal DNA was developed. The assay discriminates An. culicifacies species A and D from species B, C and E. The assay was validated using chromosomally identified specimens of An. culicifacies from different geographical regions of India representing different sympatric associations. The assay correctly differentiates species A and D from species B, C and E. The possible use of this diagnostic assay in disease vector control programmes is discussed.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Especificidad de la Especie
/
Femenino
/
Masculino
/
ADN
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Reacción en Cadena de la Polimerasa
/
Análisis de Secuencia de ADN
/
Cartilla de ADN
/
Electroforesis en Gel de Agar
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
J Biosci
Año:
2004
Tipo del documento:
Artículo
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