Bioluminometric assay of ATP in mouse brain: Determinant factors for enhanced test sensitivity.
J Biosci
;
2003 Jun; 28(4): 379-82
Artículo
en Inglés
| IMSEAR
| ID: sea-110683
ABSTRACT
Firefly luciferase bioluminescence (FLB) is a highly sensitive and specific method for the analysis of adenosine-5-triphosphate (ATP) in biological samples. Earlier attempts to modify the FLB test for enhanced sensitivity have been typically based on in vitro cell systems. This study reports an optimized FLB procedure for the analysis of ATP in small tissue samples. The results showed that the sensitivity of the FLB test can be enhanced several fold by using ultraturax homogenizer, perchloric acid extraction, neutralization of acid extract and its optimal dilution, before performing the assay reaction.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Factores de Tiempo
/
Escarabajos
/
Bioquímica
/
Encéfalo
/
Femenino
/
Adenosina Difosfato
/
Adenosina Trifosfato
/
Sensibilidad y Especificidad
/
Luciferina de Luciérnaga
/
Luciferasas
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
J Biosci
Año:
2003
Tipo del documento:
Artículo
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