Your browser doesn't support javascript.
loading
Arachidonic acid is a chemoattractant for Dictyostelium discoideum cells.
J Biosci ; 2007 Dec; 32(7): 1281-9
Artículo en Inglés | IMSEAR | ID: sea-110792
ABSTRACT
Cyclic AMP (cAMP)is a natural chemoattractant of the social amoeba Dictyostelium discoideum. It is detected by cell surface cAMP receptors. Besides a signalling cascade involving phosphatidylinositol 3,4,5-trisphosphate (PIP3), Ca2+ signalling has been shown to have a major role in chemotaxis. Previously, we have shown that arachidonic acid (AA) induces an increase in the cytosolic Ca2+ concentration by causing the release of Ca2+ from intracellular stores and activating influx of extracellular Ca2+. Here we report that AA is a chemoattractant for D. discoideum cells differentiated for 8-9 h. Motility towards a glass capillary filled with an AA solution was dose-dependent and qualitatively comparable to cAMP-induced chemotaxis. Ca2+ played an important role in AA chemotaxis of wild-type Ax2 as ethyleneglycol-bis(b-aminoethyl)-N,N,N',N'-tetraacetic acid (EGTA) added to the extracellular buffer strongly inhibited motility. In the HM1049 mutant whose iplA gene encoding a putative Ins(1,4,5)P3 -receptor had been knocked out, chemotaxis was only slightly affected by EGTA. Chemotaxis in the presence of extracellular Ca2+ was similar in both strains. Unlike cAMP, addition of AA to a cell suspension did not change cAMP or cGMP levels. A model for AA chemotaxis based on the findings in this and previous work is presented.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Quimiotaxis / Calcio / Ácido Araquidónico / Ácido Egtácico / AMP Cíclico / GMP Cíclico / Dictyostelium / Animales / Mutación Tipo de estudio: Estudio pronóstico Idioma: Inglés Revista: J Biosci Año: 2007 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Quimiotaxis / Calcio / Ácido Araquidónico / Ácido Egtácico / AMP Cíclico / GMP Cíclico / Dictyostelium / Animales / Mutación Tipo de estudio: Estudio pronóstico Idioma: Inglés Revista: J Biosci Año: 2007 Tipo del documento: Artículo