Calcium regulates the expression of a Dictyostelium discoideum asparaginyl tRNA synthetase gene.
J Biosci
;
2003 Dec; 28(6): 697-707
Artículo
en Inglés
| IMSEAR
| ID: sea-110840
ABSTRACT
In a screen for calcium-regulated gene expression during growth and development of Dictyostelium discoideum we have identified an asparaginyl tRNA synthetase (ddAsnRS) gene, the second tRNA synthetase gene identified in this organism. The ddAsnRS gene shows many unique features. One, it is repressed by lowering cellular calcium, making it the first known calcium-regulated tRNA synthetase. Two, despite the calcium-dependence, its expression is unaltered during the cell cycle, making this the first D. discoideum gene to show a calcium-dependent but cell cycle phase-independent expression. Finally, the N-terminal domain of the predicted ddAsnRS protein shows higher sequence similarity to Glutaminyl tRNA synthetases than to other Asn tRNA synthetases. These unique features of the AsnRS from this primitive eukaryote not only point to a novel mechanism regulating the components of translation machinery and gene expression by calcium, but also hint at a link between the evolution of GlnRS and AsnRS in eukaryotes.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Aspartato-ARNt Ligasa
/
Datos de Secuencia Molecular
/
Aminoacil-ARN de Transferencia
/
Secuencia de Bases
/
Homología de Secuencia de Ácido Nucleico
/
Regulación Enzimológica de la Expresión Génica
/
Calcio
/
Reacción en Cadena de la Polimerasa
/
Homología de Secuencia de Aminoácido
/
ADN Complementario
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
J Biosci
Año:
2003
Tipo del documento:
Artículo
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