Agrobacterium-mediated transformation of chickpea with alpha-amylase inhibitor gene for insect resistance.
J Biosci
;
2006 Sep; 31(3): 339-45
Artículo
en Inglés
| IMSEAR
| ID: sea-110904
ABSTRACT
Chickpea is the world's third most important pulse crop and India produces 75% of the world's supply. Chickpea seeds are attacked by Callosobruchus maculatus and C. chinensis which cause extensive damage. The alpha-amylase inhibitor gene isolated from Phaseolus vulgaris seeds was introduced into chickpea cultivar K850 through Agrobacterium-mediated transformation. A total of 288 kanamycin resistant plants were regenerated. Only 0.3% of these were true transformants. Polymerase chain reaction (PCR) analysis and Southern hybridization confirmed the presence of 4.9 kb alpha-amylase inhibitor gene in the transformed plants. Western blot confirmed the presence of alpha-amylase inhibitor protein. The results of bioassay study revealed a significant reduction in the survival rate of bruchid weevil C. maculatus reared on transgenic chickpea seeds. All the transgenic plants exhibited a segregation ratio of 31.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Fitohemaglutininas
/
Rhizobium
/
Semillas
/
Transformación Genética
/
Ingeniería de Proteínas
/
Regiones Promotoras Genéticas
/
Plantas Modificadas Genéticamente
/
Cicer
/
Lectinas de Plantas
/
Gorgojos
Idioma:
Inglés
Revista:
J Biosci
Año:
2006
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS